Question

In the diagram of pBR 322, which identifies components correctly:

• A. rop-reduced osmotic pressure
• B. Hind III, EcoRI selectable markers
• C. Am$p^R$, te$t^R$ antibiotic resistance genes
• D. ori original restriction enzyme

Answer 1

Answer: (C)

Am$p^R$, te$t^R$ antibiotic resistance genes

Given diagram is a plasmid which has following characteristics:-

Restriction sites: BamH I, Hind III, Sal I, Pvu I, Pvu II, Pst I, EcoR I, Cla I
Selectable marker: antibiotic resistance genes for ampicillin (ampR) and tetracycline
(tetR).
ORI: the origin of replication
ROP: It codes for proteins, which are involved in the process of replication of plasmid.

So the correct answer is option C.