In the diagram of pBR 322, which identifies components correctly:
A
rop-reduced osmotic pressure
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B
Hind III, EcoRI selectable markers
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C
AmpR, tetR antibiotic resistance genes
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D
ori original restriction enzyme
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Solution
The correct option is B AmpR, tetR antibiotic resistance genes Given diagram is a plasmid which has following characteristics:-
Restriction sites: BamH I, Hind III, Sal I, Pvu I, Pvu II, Pst I, EcoR I, Cla I Selectable marker: antibiotic resistance genes for ampicillin (ampR) and tetracycline (tetR). ORI: the origin of replication ROP: It codes for proteins, which are involved in the process of replication of plasmid.