In a pBR 322, a foreign DNA segment has to be introduced in the $t e t^{R}$ region. From the restriction enzymes, $B a m H I$ and $E c o R I$ , which one would you select for use? Give reason. Also mention why not the other one.

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Solution

Since restriction site for Bam HI is present in tetR region & it is a type II restriction endonuclease, it is capable of recognising short DNA sequences & cleaving them in a specific manner, whereas EcoRI is not as effective as BamHI.

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In a pBR 322, a foreign DNA segment has to be introduced in the tetR region. From the restriction enzymes, BamHI and EcoRI, which one would you select for use? Give reason. Also mention why not the other one.

Since restriction site for Bam HI is present in tetR region & it is a type II restriction endonuclease, it is capable of recognising short DNA sequences & cleaving them in a specific manner, whereas EcoRI is not as effective as BamHI.

In a pBR 322, a foreign DNA segment has to be introduced in the $t e t^{R}$ region. From the restriction enzymes, $B a m H I$ and $E c o R I$ , which one would you select for use? Give reason. Also mention why not the other one.