Question

In the diagram of pBR322, which identifies components correctly?

• A. rop–reduced osmotic pressure
• B. Hind III, EcoRI–selectable markers
• C. amp${}^R$, tet${}^R$–antibiotic resistance genes
• D. ori–original restriction enzyme

Answer 1

The correct option is C amp${}^R$, tet${}^R$–antibiotic resistance genes


pBR322 is a plasmid which has following characteristics:

Restriction sites: BamH I, Hind III, Sal I, Pvu I, Pvu II, Pst I, EcoR I, Cla I
Selectable marker: antibiotic resistance genes for ampicillin (amp${}^R$) and tetracycline (tet${}^R$)
Ori: the origin of replication
rop: It codes for proteins, which are involved in the process of replication of plasmid.