In the DNA molecule
The total amount of purine nucleotides and pyrimidine nucleotides is not always equal.
Proportion of adenine in relation to thymine varies with the organism
There are two strands which run parallel in the 5'-3' direction.
There are two strands which run antiparallel - one in 5'-3' direction and other in 3'-5' direction
The two chains have anti-parallel polarity which means one chain has the polarity 5′-3′ while the other has 3′-5′.