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Question
In the early experiments conducted by Walter Gilbert and Lydia Villa-Komaroff from Harvard University, the initial product obtained was not insulin but __________.
In the early experiments conducted by Walter Gilbert and Lydia Villa-Komaroff from Harvard University, the initial product obtained was not insulin but __________.
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Solution
The correct option is C Penicillinase-proinsulin mixture
The cDNA made from proinsulin mRNA was used for gene transfer to produce insulin by Walter Gilbert and Lydia Villa-Komaroff from Harvard University. This gene was incorporated within the penicillinase gene site and the resultant product was a mixture of proinsulin and penicillinase. Gilbert used the enzyme trypsin to remove penicillinase portion of the mixture to derive active insulin.
The cDNA made from proinsulin mRNA was used for gene transfer to produce insulin by Walter Gilbert and Lydia Villa-Komaroff from Harvard University. This gene was incorporated within the penicillinase gene site and the resultant product was a mixture of proinsulin and penicillinase. Gilbert used the enzyme trypsin to remove penicillinase portion of the mixture to derive active insulin.
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