Insect tolerant gene from Bacillus thuringiensis is introduced using Ti plasmid of _______________.

Escherichia coli

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Haemophilus influenzae

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Agrobacterium tumefaciens

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Arabidopsis Thaliana

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Solution

The correct option is C Agrobacterium tumefaciens
Plant transformation is mediated by the plant pathogen Agrobacterium tumefaciens has become the most used method for plant transformation. It is capable of transferring a particular DNA segment (T-DNA) of the tumour-inducing (Ti) plasmid into the nucleus of infected cells, where it is integrated into the host genome and transcribed, causing the crown gall disease. The insect resistant gene is inserted into the T-DNA of the plasmid which replicates in the plant to produce proteins which transform the plant into insect resistance.
So, the correct answer is option C.

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