Question

List the key tools used in recombinant DNA technology.

Answer 1

Tools are enzymes, vehicle DNA, passenger DNA. Recombinant DNA technology involves the following steps are :

• Isolation of DNA.
  
• Fragmentation of DNA by restriction endonucleases.
  
• Isolation of the desired DNA fragment.
  
• Amplification of the gene of interest.
  
• Ligation of the DNA fragment into a vector using DNA ligase.
  
• Transfer of recombination DNA into the host.
  
• Culturing the host cells on a suitable medium on a large scale.
  
• Extraction of the desired product.
  
• Downstream processing of the product as a finished product ready for marketing.