Question

Match Column I with Column II and identify the correct option.

• A. A – IV; B – I; C – II; D – III
• B. A – I; B – IV; C – II; D – III
• C. A – II; B – I; C – IV; D – III
• D. A – IV; B – I; C – III; D – II

Answer 1

The correct option is B A – I; B – IV; C – II; D – III

1. Two RNA primers complementary to both strands of DNA are added for annealing during PCR.
2. Downstream processing refers to the separation and purification of biosynthetic products, particularly pharmaceuticals. It is an essential step in the manufacture of pharmaceuticals such as antibiotics, hormones (e.g. insulin and human growth hormone), antibodies and vaccines.
3. During isolation of DNA, addition of chilled ethanol causes precipitation of purified DNA.
4. For transformation with recombinant DNA, the bacterial cells must first be made competent which means increasing the efficiency with which the DNA enters the bacterium.