Question

Match the following.

A. Plasmid	1. Breakdown of phosphodiester bond
B. Restriction enzyme	2. Initiate replication
C. DNA ligase	3. Vector
D. Origin of replication	4. Formation of phosphodiester bond

Choose the correct option.

• A. A-3, B-1, C-4, D-2
• B. A-3, B-4, C-4, D-1
• C. A-2, B-4, C-3, D-1
• D. A-1, B-3, C-2, D-4

Answer 1

The correct option is A A-3, B-1, C-4, D-2

Plasmids are circular or linear autonomously replicating extrachromosomal DNA. They are not essential for survival but they may confer selective advantage (antibiotic resistance) to the organism. As it is self-replicating and extrachromosomal in nature, it is used as a vector for the transfer of DNA into host.

A restriction enzyme is an endonuclease that identifies specific palindromic sequences in the DNA and cleaves the phosphodiester bond in both the DNA strands.

DNA ligase helps in linking the nucleotide chains by allowing the formation of phosphodiester bond.

The origin of replication is a specific sequence in the DNA at which replication starts.