Question

Match the following between the name of bacteria against their activity and choose the correct combination of the options given.

	List I		List II
A	Escherichia coli	1	Nif gene
B	Rhizobium meliloti	2	Digestive hydrocarbons of crude oil
C	Bacillus thuringensis	3	Human insulin production
D	Pseudomonas putida	4	Biocontrol of fungal disease
		5	Biodegradable insecticide

• A. A- 3, B- 1, C- 5, D- 2
• B. A- 1, B- 2, C- 3, D- 4
• C. A- 3, B- 1, C- 4, D- 2
• D. A- 1, B- 5, C- 3, D- 4

Answer 1

The correct option is A A- 3, B- 1, C- 5, D- 2

Escherichia coli is a Gram-negative bacteria. It plays an important role in the field of biotechnology, where it has served as a host organism in recombinant DNA technology. The bacterium can be grown and cultured easily. One of the first useful application of recombinant technology was the manipulation of E.coli to produce human insulin.
Rhizobium meliloti is a Gram-negative, nitrogen-fixing bacterium. It is a symbiotic bacteria lives in the association with legumes. The bacteria possesses nif genes, responsible for the nitrogen fixation.
Bacillus thuringensis is a soil dwelling bacteria. It has been used as a biological pesticide. During spore formation, the bacteria produces crystal proteins called as endotoxins which have insecticidal action.
Crude oil is a complex mixture of hydrocarbons. Several strains of bacterium Pseudomonas putida were known to produce enzymes that degrade these hydrocarbons, i.e., the bacteria ate the hydrocarbons, nourishing themselves and reproducing.

So, the correct answer is option A.