The correct option is D All of these
Conventional breeding methods like hybridization and selection among various crop varieties and wild relatives is one of the methods for breeding crops. The constraint in this method is the limited number of disease resistance genes. Mutation can be induced in the plants to create the desired genetic variation resulting in the formation of a new characteristic that imparts disease resistance which is not found elsewhere. Other methods like selection among somaclonal variants and genetic engineering are also useful in developing disease-resistant varieties.