Question

Mohit followed the following procedure for staining the temporary mount of leaf peel (material) on slide.

(i) To put a single drop of glycerine on material.

(ii) To put a single drop of stain on material and wash it with water.

(iii) Cover the material with coverslip.

(iv) Observe the slide under microscope.

The correct sequence is

• A. (i), (ii), (iii), (iv)
• B. (iv), (iii), (ii), (i)
• C. (ii), (i), (iii). (iv)
• D. (iii), (ii), (iv), (i).

Answer 1

The correct option is C

(ii), (i), (iii). (iv)

The correct option is option (C)

Explanation for the correct option:

1. To stain a leaf peel, it needs to be stained, followed by washing the dye.
2. Glycerin is added to prevent cells from drying and maintain an isotonic environment.
3. Finally, a coverslip is used to view the sample under the microscope.

Explanation for the incorrect options:

Option (A): This option is incorrect as glycerin is not added before the stain on the leaf peel.

Option (B): The leaf peel cannot be mounted before staining, and thus this option is incorrect.

Option (D): This option is incorrect as the dye is added before placing the coverslip.

The correct sequence of the procedure is (ii), (i), (iii), (iv) option (C)