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What is PCR

Name the bact...

Question

Name the bacterium that yields a thermostable DNA polymerase.

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Solution

The bacterium that yields a thermostable DNA polymerase is Thermus aquaticus

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Q. Use of a thermostable DNA polymerase from the bacterium, Thermus aquatics, made it possible to generate a billion copies of DNA in a very short time using a process. Name the process.

Q. Why is a thermostable DNA polymerase needed in amplification/genetic engineering?

Q. Given an example of the source of thermostable enzyme DNA polymerase.

Q. In addition to Taq polymerase enzyme which other thermostable DNA polymerases have been isolated to be used in polymerase chain Reaction (PCR)?

Q. Rearrange the following in the current sequences to accomplish an important biotechnological reaction.
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA

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