Question

Of the following compounds, which would you expect to elute last from a reverse-phase liquid chromatography column?

• A. Methanol
• B. n-Propanol
• C. n-Butanol
• D. n-Pentanol

Answer 1

Answer: (D)

n-Pentanol

With increase in the number of carbon chain, its non-ploar nature increases;

in reverse phase liquid chromatography stationary phase is non-polar in nature, so n-pentanol having more non-polar character and elutes last.