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Introduction to Biotechnology

what are reco...

Question

what are recombinants and non recombinants DNA

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Solution

Recombinant DNA are DNA molecules formed in the laboratory by techniques of genetic recombination, bringing together DNA from various sources which can not be created in a biological organism otherwise.
Foreign DNA which is of no use is called non recombinant DNA.

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Q. What is recombinant DNA technology?

Q. After insertion of DNA segment within the sequence of Z-gene of bacteria, they are grown on chromogenic substrate. After the growth of bacterial colonies, they are identified as
i) Recombinants if colonies are blue- coloured.
ii) Recombinants if colonies are white coloured.
iii) Non-recombinants if colonies are blue- coloured.
iv) Non-recombinants if colonies are white coloured.

Q. Define recombinant DNA.

Q. If a person obtains transformants by inserting a recombinant DNA within the coding sequence of enzyme -galactosidase, he will separate out recombinants from non-recombinants by which of the following observations?

Q. How many of the following statement(s) regarding insertional inactivation of lacZ gene are incorrect?

1. lacZ gene remains active in non-recombinant transformed bacteria.
2. X-gal gets hydrolysed and produces a blue product in non-recombinant transformed bacteria.
3. Bacterial colonies which are transformed with recombinant DNA appear white.
4. 𝛃-Galactosidase is produced in recombinant bacteria.
5. Bacterial colonies which are transformed with recombinant DNA appear blue.

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