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What is the d...

Question

What is the difference between a recognition site and a restriction site?

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Solution

Recognition site is a sequence on a DNA to which endonuclease binds and cut the DNA at that site whereas restriction site is a sequence of DNA to which restriction enzymes bind to cut the DNA at that site.

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Q. How many of the following statements are correct?
I. If a linear DNA molecule is digested with a restriction enzyme at four recognition sites, 4 fragments would be produced.
II. If a closed circular DNA molecule is digested with a restriction enzyme at six recognition sites, 6 fragments would be produced.
III. If a linear DNA molecule is digested with a restriction enzyme at four recognition sites on the DNA, 5 fragments would be produced.
IV. If a closed circular DNA molecule is digested with a restriction enzyme at six recognition sites, 7 fragments would be produced.

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