Question

pBR322 is a:

• A. Radioactive isotope of P
• B. Artificial plasmid, used as a vector for cloning gene in E.coli
• C. Radioactive bromine used as a genetic marker for the study of metabolic pathway
• D. A variety of maize

Answer 1

The correct option is B Artificial plasmid, used as a vector for cloning gene in E.coli

pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. Created in 1977 in the laboratory of Herbert Boyer at University of California. It is a circular double-stranded molecule 4,361 base pairs in length. pBR322 contains the genes for resistance to ampicillin and tetracycline and can be amplified with chloramphenicol.