Question

pBR322 was the first artificial cloning vector to be constructed. What does "BR" stands for?

• A. Bacteriophage and Recombinant
• B. Boliver and Rodriguez
• C. Boyer and Replicative
• D. None of these

Answer 1

Answer: (B)

Boliver and Rodriguez

pBR322 was the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez. It is widely used in gene cloning experiments.

• In pBR322 plasmid vector,
• p - denotes that it is a plasmid;
• BR - stands for Boliver and Rodriguez who constructed this plasmid;
• 322 - Is a number given to distinguish this plasmid from others developed in the same laboratory.
• E coli cloning vector pBR 322 has restriction sites (HindIII, EcoRI, BamHl, SaII, PvuII, PstI, ClaI), ori (origin of replication) and antibiotic resistance genes ($am{p}^R$ and $te{t}^R$). rop codes for the proteins involved in the replication of the plasmid.

So, the correct answer is 'Boliver and Rodriguez'.