Plasmid pBR322 has Pst I restriction enzyme site within gene amp^Rthat confers ampicillin resistance. If this enzyme is used for inserting a gene for ß-galactoside production and the recombinant plasmid is inserted in an E. coli strain

it will be able to produce a novel protein with dual ability.

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it will not be able to confer ampicillin resistance to the host cell.

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the transformed cells will have the ability to resist ampicillin as well as produce ß-galactoside.

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it will lead to lysis of host cell.

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Solution

The given question is a case of insertional inactivation. Insertional inactivation refers to the inactivation of a gene due to the insertion of a DNA segment within its sequence.
When a gene coding for ß-galactoside is inserted into the plasmid pBR322 at the Pst I site within the amp^Rgene, the ampicillin resistance gene will be inactivated as a result of which, it will fail to confer ampicillin resistance to its host. However, ß-galactoside will be produced in the host cell as the gene encoding the same will be intact.

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A. Recombinants will lose ampicillin resistance.
B. Recombinants will grow on a medium containing ampicillin but not on that containing tetracycline.
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D. Recombinants will grow on a medium containing tetracycline but not on that containing ampicillin.

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Plasmid pBR322 has Pst I restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for ß-galactoside production and the recombinant plasmid is inserted in an E. coli strain

Plasmid pBR322 has Pst I restriction enzyme site within gene amp^Rthat confers ampicillin resistance. If this enzyme is used for inserting a gene for ß-galactoside production and the recombinant plasmid is inserted in an E. coli strain