Question

Probe used initially in DNA fingerprinting is

• A. Single stranded RNA
• B. Mini satellite
• C. 19 base long oligonucleotide
• D. All of the above

Answer 1

Answer: (B)

Mini satellite

DNA fingerprint of an individual is essentially a southern blot of his DNA digested with an endonuclease and probed with a radioactive DNA probe. The probes used for DNA fingerprinting are usually prepared from minisatellite of microsatellite DNA. Microsatellites are also known as simple sequence repeats or short tandem repeats (STRs), are repeating sequences of 2-5 base pairs of DNA.

So, the correct answer is option B.