Question

Process used for amplication or multiplication of DNA for fingerprinting is:

• A. Nesslerisation
• B. Northern blotting
• C. Polymerase chain reaction
• D. Southern blotting

Answer 1

The correct option is C

Polymerase chain reaction

Polymerase chain reaction is a technique of synthesizing multiple copies of the desired gene (DNA) in vitro.

Nesslerisation is the classical colorimetric method for ammonia determination.

Southern blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blot analysis reveals information about DNA identity, size and abundance.

Northern blot is a technique used in molecular biology research to study gene expression by detection of RNA in a sample. Northern blot analysis reveals information about RNA identity, size and abundance.