Protoplast does not include

Plasma membrane

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Cell wall

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Nucleus

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Cytoplasm

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Solution

The correct option is A Cell wall
Protoplast does not include cell wall.
Protoplasts refer to the living cell (bacterial cell or plant cell) which lacks a cell wall (removed by enzymatic or mechanical means).
The cells are surrounded by the cell membrane or plasmalemma.
So, the correct answer is 'Cell wall'.

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