The correct option is D J. Lister
Joseph Lister, 1878, was first to obtain the pure culture of bacteria by serial dilution of liquid medium containing a mixture of bacterium; option D is correct. R. Koch developed the liquidefeable solid culture medium to facilitate transfer of pure culture to other medium and discovered the causative agent of tuberculosis. Option A is incorrect. L. Pasteur followed the pure culture technique as described by R. Koch and discovered the causative agent of chicken cholera; option B is incorrect. A. Leeuwenhoek formed single lensed microscope to observe the tiny organisms which were then named as microorganisms. Thus, the correct answer is option D.