Question

Recombinant DNA technology can be accomplished only if we have the following key tools i.e., reaction enzymes, polymerase enzyme, ligases and vectors.
State the functions of:
(a) Ligases
(b) Restriction enzymes

Answer 1

DNA ligase enzyme joins two DNA fragments together via phosphodiester bond between 3’ hydroxyl at the end of one fragment and a 5’ phosphate at the end of another fragment.

Restriction endonucleases are called biological scissors because they cleave (cut) DNA at or near specific recognition sequences (restriction sites). They act as an important tool for gene modification and are widely used