Question

Recombinant DNA technology involves several steps in specific sequence. Find out the correct sequence.

a. Fragmentation of DNA

b. Culturing the host cells in a medium at large scale

c. Ligation of DNA fragment into a vector

d. Extraction of the desired product

e. Isolation of DNA

f. Isolation of desired DNA fragment

g. Transferring the recombinant DNA into the host

• A. e → a → f → g → c → b → d
• B. e → f → a → c → g → b → d
• C. e → a → f → c → g → b → d
• D. a → e → c → f → g → d → b

Answer 1

The correct option is C e → a → f → c → g → b → d

Steps in rDNA technology

Recombinant DNA technology involves the following steps:-

• Isolating the DNA from the respective organism by breaking the cells (e).
• Fragmentation of the DNA using restriction enzymes (a).
• Isolating the DNA fragments that contain the desired gene (f).
• Linking the DNA fragment containing the desired gene with the vector DNA such as plasmid using DNA ligase enzyme (c).
• Transferring the rDNA (desired DNA fragment + vector DNA) to the host cell for gene cloning (g).
• Growing the host cell on the suitable cultured media to produce the clones of the desired gene (b).
• Extraction of the product after culturing the host cell (d).

Final answer

(d) e → a → f → c → g → b → d