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Question

'Restriction' in restriction enzyme refers to:


A
Cleaving of phosphodiester bond in DNA by the enzyme
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B
Cutting of DNA at specific position only
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C
Prevention of the multiplication of bacteriophage in bacteria
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D
All of the above
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Solution

The correct option is B Cutting of DNA at a specific position only


The restriction enzymes are called 'molecular scissors' and are responsible for cutting DNA. Restriction enzyme belongs to a class of enzymes called nucleases.
They are of two types
(i) Exonucleases: Cut DNA at the ends
(ii) Endonucleases: Make cuts at specific positions within the DNA.

These enzymes are present in bacteria to provide a type of defense mechanism called the 'restriction-modification system'. This system consists of two components, restriction enzyme, and modification enzyme. The term 'restriction' refers to the function of these enzymes in restricting the propagation of foreign DNA of bacteriophage in host bacterium, i.e., cutting of DNA at a specific position only.

Nuclease is an enzyme capable of cleaving the phosphodiester bonds between monomeric units of nucleic acids.


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