The correct option is A Direction of RNA synthesis - 5' - 3' ; Direction of reading of the template DNA strand - 3' - 5'
Transcription is the process of synthesizing RNA from a DNA template. RNA polymerase synthesizes the RNA strand and it takes place only in the direction 5' - 3'. Since the newly synthesised strand is always complementary to the template strand, which is in the direction 3' - 5'.