Question

Select the incorrect Statement regarding DNA replication with respect to the template strand

• A. Leading strand is formed in 5 $\to$ 3 direction.
• B. Okazaki fragments are formed in 5 $\to$ 3 direction.
• C. DNA polymerase catalyses polymerisation in 5 $\to$ 3 direction.
• D. DNA polymerase catalyses palymerisation in 3 $\to$ 5 direction.

Answer 1

The correct option is C DNA polymerase catalyses polymerisation in 5 $\to$ 3 direction.

Since DNA polymerase requires a free 3' OH group for initiation of synthesis, it can synthesize in only one direction by extending the 3' end of the preexisting nucleotide chain. Hence, DNA polymerasemoves along the template strand in a 3'–5' direction, and the daughter strand is formed in a 5'–3' direction.

So, the correct answer is 'DNA polymerase catalyses polymerisation in 5

→→ 3 direction'