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Blunt and Sticky Ends

Selective mar...

Question

Selective markers in plasmids are used to

A

Identify cancer cells

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B

Identify antibiotics

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C

Identify recombinants from non recombinants

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D

None of these

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Solution

The correct option is C
Identify recombinants from non recombinants

Plasmid is an extra chromosomal genetic material which has selective markers like ampicillin resistance and tetracycline genes. They are used to identify the recombinants from non-recombinants.

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Q. Identify the correct sequence of steps in the construction of recombinant DNA.

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use restriction enzymes

(2)

(3)

remove plasmid from parent bacterium

(4)

introduce plasmid into new host bacterium.

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ii) Recombinants if colonies are white coloured.
iii) Non-recombinants if colonies are blue- coloured.
iv) Non-recombinants if colonies are white coloured.

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Blunt and Sticky Ends

Standard VII Biology

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