Separation and purification by filtration, centrifugation of culture broth are a part of
Separation and purification by filtration, centrifugation of culture broth are a part of
The correct option is D Downstream processing
Downstream processing (DSP) is the series of operations used in biotechnology where the desired homogenous and purified protein is isolated from biological materials such as cells, tissue culture fluid, or plant tissues. As these products are embedded initially in a biological matrix with many unwanted molecules, DSP employs a multistep process that incrementally increases the purity of the target by exploiting the physical and chemical properties of the desired product that make it distinct from contaminants. DSP also involves harvesting and clarification steps to remove bulk contaminants such as particulates, carbohydrates, and oils followed by capturing and polishing steps that refine the feed stream until only the target product remains.
The upstream bioprocess steps involve development of inoculum, optimising the culture medium and inoculum so that culture is made ready to produce the desired product in topmost quality and quantity. Later the desired product is obtained by subjecting it to the downstream processes.
Screening of recombinants is done to select the colonies which are capable of producing high quality and quantity of products.
Downstream processing (DSP) is the series of operations used in biotechnology where the desired homogenous and purified protein is isolated from biological materials such as cells, tissue culture fluid, or plant tissues. As these products are embedded initially in a biological matrix with many unwanted molecules, DSP employs a multistep process that incrementally increases the purity of the target by exploiting the physical and chemical properties of the desired product that make it distinct from contaminants. DSP also involves harvesting and clarification steps to remove bulk contaminants such as particulates, carbohydrates, and oils followed by capturing and polishing steps that refine the feed stream until only the target product remains.
The upstream bioprocess steps involve development of inoculum, optimising the culture medium and inoculum so that culture is made ready to produce the desired product in topmost quality and quantity. Later the desired product is obtained by subjecting it to the downstream processes.
Screening of recombinants is done to select the colonies which are capable of producing high quality and quantity of products.
