1
Question
Silencing of mRNA or RNA interference has been used in development of plants resistant to
Silencing of mRNA or RNA interference has been used in development of plants resistant to
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Solution
The correct option is D Nematodes
RNA interference (RNAi) or silencing of RNA is a method used to prevent the expression of a gene.
Nematode parasites like the root-knot worm, Meloidogyne incognita, attack the root cells of plants (tobacco) which then form large galls or knots, damaging the crop and reducing the yield.
Gene from M. incognita can be cloned in the Ti plasmid and can be introduced in the tobacco plant using the Agrobacterium vector. It is done in such a way that both the sense and antisense RNA get expressed which are complementary to each other. This forms double stranded RNA.
Cellular machinery processes the long double stranded RNA into shorter stretches. This dsRNA is taken up by the nematode while it feeds on the host plant parts and RNAi is initiated. The particular gene(s) targeted (usually the gene(s) essential development) is silenced. Therefore, the nematode cannot grow in the host plant and the plant gains pest resistance.
Hence the correct option is
d. Nematodes
RNA interference (RNAi) or silencing of RNA is a method used to prevent the expression of a gene.
Nematode parasites like the root-knot worm, Meloidogyne incognita, attack the root cells of plants (tobacco) which then form large galls or knots, damaging the crop and reducing the yield.
Gene from M. incognita can be cloned in the Ti plasmid and can be introduced in the tobacco plant using the Agrobacterium vector. It is done in such a way that both the sense and antisense RNA get expressed which are complementary to each other. This forms double stranded RNA.
Cellular machinery processes the long double stranded RNA into shorter stretches. This dsRNA is taken up by the nematode while it feeds on the host plant parts and RNAi is initiated. The particular gene(s) targeted (usually the gene(s) essential development) is silenced. Therefore, the nematode cannot grow in the host plant and the plant gains pest resistance.
Hence the correct option is
d. Nematodes
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