Question

Solve this:

154. The colonies of non-recombinant bacteria appers blue in contrast to white colonies of recombinant bacteria because of :-

(1) Non-recombinant bacteria containing beta- galactosidase
(2) Insertional inactivation of alpha- galactosidase in non-recombinant bacteria
(3) Insertional inactivation of alpha- glactosidase in recombinant bacteria
(4) Inactivation of glycosidase enzyme in recombinant bacteria

Answer 1

Dear student,
Answer is 3) insertional inactivation of alpha galactosidase in recombinant bacteria.
Non recombinant bacteria forms blue colonies due to the activation of the enzyme alpha galactosidase which hydrolyses alpha glyctosyl moiety from glycolipid and glycoproteins. But when recombinant bacteria is formed, the gene (GLA) encoding alpha galactosidase enzyme becomes inactivated due to which white colony of bacteria is formed.

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