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Antibiotics

The colonies ...

Question

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of

A

Insertional inactivation of alpha galactosidase in recombinant bacteria

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B

Inactivation of glycosidase enzyme in recombinant bacteria

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C

Non-recombinant bacteria containing beta galactosidase

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D

Insertional inactivation of alpha galactosidase in non-recombinant bacteria

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Solution

The correct option is A Insertional inactivation of alpha galactosidase in recombinant bacteria
The lungs of child starts working only after birth at that time the lung tissue are filled with air making it lighter and hence it floats on water. If the child was still born the lungs would have been heavier due to absence of air and would have settled down on the bottom. But it hasn't happened. So victim is father.

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Q. The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because

Q. The colonies of recombinant bacteria appear white in contrast to blue colonies of nonrecombinant bacteria because of

Q. How many of the following statement(s) regarding insertional inactivation of lacZ gene are incorrect?

1. lacZ gene remains active in non-recombinant transformed bacteria.
2. X-gal gets hydrolysed and produces a blue product in non-recombinant transformed bacteria.
3. Bacterial colonies which are transformed with recombinant DNA appear white.
4. 𝛃-Galactosidase is produced in recombinant bacteria.
5. Bacterial colonies which are transformed with recombinant DNA appear blue.

Q. After insertion of DNA segment within the sequence of Z-gene of bacteria, they are grown on chromogenic substrate. After the growth of bacterial colonies, they are identified as
i) Recombinants if colonies are blue- coloured.
ii) Recombinants if colonies are white coloured.
iii) Non-recombinants if colonies are blue- coloured.
iv) Non-recombinants if colonies are white coloured.

Q. (a) A recombinant vector with a gene of interest inserted within the gene of

α

-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones.
(b) Why is this method of selection referred to as "insertional inactivation"?

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