Some foreign DNA fragment is attached to Cla I site of pBR322. This recombinant vector is used to transition Escherichia coli. Then on two different media, one containing ampicillin and the other containing the recombinant vector will

grow on both tetracycline and ampicillin containing media

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not grow on either tetracycline containing or ampicillin containing media

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grow on tetracycline but not on ampicillin containing medium

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grow on ampicillin but not on tetracycline containing medium

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Solution

The correct option is A grow on both tetracycline and ampicillin containing media
pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. Some foreign DNA fragment is attached to Cla I site of pBR322. This recombinant vector in used to transition Escherichia coli. Then on two different media, one containing ampicillin and the other containing tetracycline, the transformed cells containing the recombinant vector will grow on both, tetracycline and ampicillin containing media because Cla–I site is neither present on ${amp}^{R} gene$ nor on ${tet}^{R} gene$ .

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Q. Some foreign DNA fragment is attached to Cla I site of pBR322. This recombinant vector is used to transition Escherichia coli host cells. The cells subjected to transformation are plated on two different media-one containing ampicillin and the other containing tetracycline. The transformed cells containing the recombinant vector

Q. Some of foreign DNA fragment is attached to

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site of

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