Some of foreign DNA fragment is attached to $C l a I$ site of $p B R 322.$ This recombinant vector is used to transition Escherichia coli host cells. The cells subjected to transformation are plates on two different media - one containing ampicillin and the other containing tetracycline. They transformed cells containing the recombinant vector :

Will grow on both tetracycline and ampicillin containing media.

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Will not grow on both tetracycline and ampicillin containing media.

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Will grow on tetracycline, but not on ampicillin containing media.

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Will grow on ampicillin, but not on tetracycline containing media.

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Solution

The correct option is A Will grow on both tetracycline and ampicillin containing media.
Answer is option A i.e. "Will grow on both tetracycline and ampicillin containing media"
pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. Plasmid pBR322 confers resistance to ampicillin and tetracycline. Since, the foreign DNA fragment is attached to Cla 1 site, the transformant will be observed on both the plates as Cla 1 site is not a part of tetracycline and ampicillin resistant genes.

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