Question

Some of the common steps involved in the production of humulin are given below. Choose the correct sequence.
I. Synthesis of the gene (DNA) for human insulin artificially.
II. Culturing of recombinant E. coli in bacteria.
III. Purification of humulin.
IV. Insertion of human insulin gene in plasmid DNA.
V. Introduction of recombinant plasmid into E. coli.
VI. Extraction of recombinant gene product from E. coli.

• A. II, I, IV, Ill, V, VI
• B. I, III, V, VI, II, IV
• C. I, IV, V, II ,VI, III
• D. I, V, IV, II ,VI, III

Answer 1

Answer: (C)

I, IV, V, II ,VI, III

Humulin, a synthetic version of Insulin, is produced using modern genetic engineering technology. The following steps are involved in the production of humulin:

I. Synthesis of the gene (DNA) for human insulin artificially.

II. Insertion of human insulin gene in plasmid DNA.

III. Introduction of recombinant plasmid into Escherichia coli.

IV. Culturing of recombinant E. coli in bacteria.

V. Extraction of a recombinant gene product from E. coli.

VI. Purification of humulin.