Some of the steps involved in the production of humilin are given below. Arrange them in the correct sequence and select the correct option.
(i) Synthesis of gene (DNA) for human insulin artificially
(ii) Culturing recombinant E.coll in bioreactors
(iii) Purification of humilin
(iv) Insertion of human insulin gene into plasmid
(v) Introduction of recombinant plasmid into E.coli
(vi) Extraction of recombinant gene product from E.coli.