Bacterial Synthesis of Insulin (Human)

Q. The number of disulphide bonds present in between the A and B chains of insulin are

1. Two
2. One
3. Three
4. Four

Q.

What are the disadvantages of fermentation?

Q. The first human hormone produced by recombinant DNA technology is:

1. Insulin
2. Estrogen
3. Progesterone
4. Thyroxin

Q.

Choose the incorrect statement about insulin-

1. It is produced in the pancreas

2. Regulates the growth and development of the body

3. Regulates blood sugar level

Q.

How is sugar level controlled in the body?

Q. Assertion: Fermentation occurs by the incomplete oxidation of glucose.
Reason: Yeast forms ethanol & CO2 from pyruvic acid.

1. Both A and R are true and R is the correct explanation of A.
2. Both A and R are true but R is not a correct explanation of A
3. A is true but R is false
4. A and R are false
   

Q. What is the hormone that suppresses hunger?

1. Ghrelin
2. Melanin
3. Thyroxine
4. Leptin

Q. The term ‘Humulin’ is used for

1. Hydrolytic enzyme
2. Isoenzyme
3. Human insulin
4. Antibiotic

Q.

Fermentation carried out by yeast is called as _________ .

1. pyruvic fermentation

2. basic fermentation

3. lactic acid fermentation

4. alcoholic fermentation

Q. How many protein chains are present in insulin?

1. 1
2. 2
3. 4
4. 3

Q.

What can kill yeast during fermentation?

Q. Match the following
$\begin{array}{cc}ColumnI& ColumnII\\ \text{A. Humulin}& \text{i. Possesses 21 amino acids.}\\ \text{B. A-peptide}& \text{ii. Extra stretch present in pro-insulin.}\\ \text{C. B-peptide}& \text{iii. First genetically engineered hormone.}\\ \text{D. C-peptide}& \text{iv. Connects peptide chains.}\\ \text{E. Disulphide Bonds}& \text{v. Possesses 30 amino acids.}\end{array}$

1. A-v, B-iv, C-iii, D-ii, E-i
2. A- iii, B-i, C-v, D-ii, E-iv
3. A-ii, B-iv, C-i, D-iii, E-v
4. A-iv, B-iii, C-ii, D-i, E-v

Q.

State whether the following statement is true or false.

The four nitrogenous bases in the DNA are Guanine, Thiamine, Adrenaline, and Cytosine.

1. True
2. False

Q.

What were the problems with extraction of insulin from animals?

1. The insulin from animals did not work for humans.

2. The efficiency of animal insulin was less.

3. Extraction of animal insulin was very expensive.

4. The amount of insulin extracted from a single animal was not sufficient to meet the demand for insulin.

Q.

Name the hormone secreted by beta cells of islets of Langerhans

Q. Explain the steps involved in the production of genetically engineered insulin.

Q. In 1921, Banting and Best isolated insulin from the pancreas of ___.

1. pigs
2. calves
3. dogs
4. rabbits

Q. Which of the following is responsible for the production of insulin in human body?

1. Nephrons
2. Islets of Langerhans
3. Mylein sheath
4. Bowman's capsule

Q. Assertion :Insulin is an important life saving drug for diabetic patients. Reason: It is now possible to produce insulin by using recombinant DNA technology.

1. If both Assertion and Reason are correct and Reason is the correct explanation of Assertion.
2. If both Assertion and Reason are correct, but Reason is not the correct explanation of Assertion.
3. If Assertion is correct but Reason is incorrect.
4. If Assertion is incorrect but Reason is correct.

Q.

Name the following-

The hormone is secreted by islets of Langerhans.

Q. First hormone obtained from genetically engineered bacteria is

1. Adrenaline
2. Thyroxine
3. Insulin
4. Testosterone

Q.

Identify the incorrect statement(s) regarding hormones from non-endocrine glands:

I. An unorganized system which works on a negative feedback mechanism.

II. Includes major parts or organs like heart, kidney, epithelial tissues, etc.

III. Most of them are very much related to digestion or excretion.

IV. All the hormones of the system are steroids and their derivatives.

1. IV

2. III

3. II

4. I

Q. The first human hormone produced by recombinant DNA technology is?

1. insulin
2. estrogen
3. thyroxin
4. progesterone

Q.

What was the primary challenge for the production of insulin using rDNA technology?

Q. Some of the steps involved in the production of humilin are given below. Arrange them in the correct sequence and select the correct option.
(i) Synthesis of gene (DNA) for human insulin artificially
(ii) Culturing recombinant E.coll in bioreactors
(iii) Purification of humilin
(iv) Insertion of human insulin gene into plasmid
(v) Introduction of recombinant plasmid into E.coli
(vi) Extraction of recombinant gene product from E.coli.

1. (ii), (i), (iv), (iii), (v), (vi)
2. (i), (iii), (v), (vi), (ii), (iv)
3. (i), (iv), (v), (ii), (vi), (iii)
4. (iii), (v), (ii), (i), (vi), (iv)

Q. Write a brief note on Genetically Engineered Insulin.

Q. Who discovered insulin and from where?

Q. Human insulin is commercially produced from a transgenic species of the ______ genus.

1. Rhizobium
2. Saccharomyces
3. Escherichia
4. Mycobacterium

Q. The insulin prepared through genetic engineering is called as

1. Micro insulin
2. Human insulin
3. Bioinsulin
4. Humulin

Q. Plasmid is boon to biotechnology (with reference to production of insulin).

1. True
2. False