Question

Some of the steps involved in the production of humulin are given below. Choose the correct sequence.
(i) Synthesis of gene (DNA) for human insulin artificially.
(ii) Culturing recombinant E.coli in bioreactors.
(iii) Purification of humulin.
(iv) Insertion of human insulin gene into plasmid.
(v) Introduction of recombinant plasmid into E.coli.
(vi) Extraction of recombinant gene product from E.coli.

• A. (ii), (i), (iv), (iii), (v), (vi)
• B. (i), (iii), (v), (vi), (ii), (iv)
• C. (i), (iv), (v), (ii), (vi), (iii)
• D. (iii), (v), (ii), (i), (vi), (iv)

Answer 1

Answer: (C)

(i), (iv), (v), (ii), (vi), (iii)

The steps involved in the production of humulin are:
(I) RNA is collected for cells that produce insulin.
(ii) The RNAs are converted to cDNA by reverse transcriptase.
(iii) The cDNA is fused to a strong promoter.
(iv) The fused gene is inserted into a microorganism.
(v) The microorganisms are grown under conditions where they will produce protein.
(vi) The protein is separated from the cells and medium and purified.

Thus, the correct answer is (C).