Question

Some of the steps involved in the production of humulin are given below. Choose the correct sequence.
(i) Synthesis of gene (DNA) for human insulin artificially.
(ii) Culturing recombinant E.coli in bio reactors.
(iii) Insertion of human insulin gene into a plasmid.

(iv) Introduction of recombinant plasmid into E.coli.
(v) Extraction of recombinant gene product from E.coli.

• A. (i), (iii), (iv), (ii), (v)
• B. (iii), (v), (ii), (i), (iv)
• C. (ii), (i), (iv), (iii), (v)
• D. (i), (iii), (v), (ii), (iv)

Answer 1

The correct option is A (i), (iii), (iv), (ii), (v)

Steps involved in the production of humulin hormone are as follow:

• Firstly, the synthesis of DNA for human insulin takes place.
• This gene of interest is then inserted into a plasmid. Thus, forming a recombinant DNA plasmid.
• The recombinant DNA plasmid then introduced into the host cell, which is E. coli in this case.
• This host cell having recombinant plasmid is then cultured into a bio-reactor to produce the required humulin protein.
• The protein (i.e., the recombinant gene product) from E.coli. is extracted from the culture by using down stream processing technique.

Thus, the correct answer is (A).