Question

Sort the stepwise processes in rDNA technology.

• A. Identify GOI
• B. Copies of GOI
• C. Insertion of GOI
• D. Screening
• E. Cloning

Answer 1

The steps in rDNA technology are as follows:
Step 1 - The desired gene, that is the ‘gene of interest’ (GOI) is identified and isolated.
Step 2 - Multiple copies of the ‘gene of interest’ are made using techniques such as polymerase chain reaction (PCR).
Step 3 - Gene of interest is incorporated into a delivery vehicle termed as a vector, which will then insert it into a host.
Step 4 - Gene of interest is delivered into a host living organism so that it can replicate and translate the protein of choice.
Step 5 - Screening is done to identify the organism that contains the rDNA.
Step 6 - Cloning of the organism containing the rDNA and extraction of the desired protein for human use is carried out.