Question

Sterilization of tissue culture medium is done by

• A. Autoclaving of medium at 120${}^o$ C for 15 minutes
• B. Filtering the medium through fine sieve
• C. Mixing the medium with antifungal agents
• D. Keeping the medium at - 20${}^o$C

Answer 1

The correct option is A Autoclaving of medium at 120${}^o$ C for 15 minutes

Plant tissue culture media are generally sterilized by autoclaving at 120${}^o$C and 1.05 kg/cm${}^2$ (15-20 psi). The time required for sterilization depends upon the volume of medium in the vessel. The minimum times required for sterilization of different volumes of medium is 15 min.

So, the correct answer is 'Autoclaving of medium at 120

oo C for 15 minutes'