Question

Sterilization of tissue culture medium is done by

• A. Autoclaving of medium at ${120}^0$C for 15 minutes
• B. Filtering the medium through fine sieve
• C. Mixing the medium with antifungal agents
• D. Keeping the medium at $-{20}^{0}C$

Answer 1

Answer: (A)

Autoclaving of medium at ${120}^0$C for 15 minutes

Tissue culture is a method to propagate desired plants in a controlled condition and called as in vitro method. In this experiment, a culture medium is used as a source of nutrients which contain growth regulators also. The culture medium being also conducive for the growth of bacteria and fungi, this is sterilized to avoid any contamination by microorganisms. Sterilization of the tissue culture medium is done through autoclaving at 120${}^0$C for 15 minutes. In this method of sterilization, moist heat is used to kill microorganisms.

So, the correct answer is option A.