Question

Stirred-tank bioreactors have been designed for

• A. Availability of oxygen throughout the process
• B. Ensuring anaerobic conditions in the culture vessel
• C. Purification of the product
• D. Addition of preservatives to the product

Answer 1

The correct option is A

Availability of oxygen throughout the process

The correct option is A.

Explanation of the correct option:

1. Aeration in the culture media is achieved using stirred tank bioreactors. Inside the culture, there are enormous blades that are constantly spinning.
2. The air mixes with the culture due to the movement of the blades, resulting in proper aeration.
3. The availability of oxygen for a specific metabolic process is increased when there is more air in the culture.

Explanation of the incorrect options:

Option B:

1. If the system is isolated from the atmosphere, the internal culture does not come into contact with the outer atmosphere, the anaerobic state can be maintained in a culture vessel.
2. Microorganisms that develop in anaerobic circumstances keep this type of habitat going.

Option C:

1. After downstream processing, purification takes place.
2. It can only happen after the end products have been removed from the tank and have undergone chemical and physical changes.

Option D:

1. Preservatives are only added to a product if it needs to be kept for an extended period of time.
2. The continual rotation of the blood in the bioreactor, on the other hand, has nothing to do with the addition of preservatives.

Final answer: Stirred-tank bioreactors have been designed for availability of oxygen throughout the process.