Question

Study the following lists.
List-I List-II
(A) Colony hybridization (I) Transfer of recombinant DNA into a host cell
(B) Gel electrophoresis (II) Selection of cells containing the desired gene
(C) Gradient centrifugation (III) Separation of DNA fragments
(D) Polymerase chain reaction (IV) Purification of DNA
(V) Gene cloning in thermocycler
The correct match is

• A. (A)- (IV), (B)- (V), (C)- (II), (D)- (III)
• B. (A)- (I), (B)- (II), (C)- (III), (D)- (IV)
• C. (A)- (II), (B)- (III), (C)- (IV), (D)- (V)
• D. (A)- (III), (B)- (IV), (C)- (V), (D)- (II)

Answer 1

Answer: (C)

(A)- (II), (B)- (III), (C)- (IV), (D)- (V)

Colony hybridisation technique is a genetics lab technique used to identify which colonies of bacteria on an agar plate contain a particular sequence of DNA or a particular gene.

Gel electrophoresis is a method for separation and analysis of macromolecules like DNA, RNA and proteins and their fragments, based on their size and charge.

Gradient centrifugation is the ultracentrifugation of substances in concentrated solutions of cesium salts or of sucrose; at equilibrium, the medium exhibits a concentration (hence density) gradient increasing in the direction of centrifugal force and the substances of interest collect in layers at the levels of their densities.

The polymerase chain reaction (PCR) is a process used in molecular biology to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. The process is carried out in the equipment known as thermocycler.

Thus, the correct answer is option C.