Question

Study the following statements regarding recombinant DNA technology and select the incorrect ones

(i) Taq polymerase extends the primers using the nucleotides provided in the reaction

(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology

(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis

(iv) Transformation is a procedure through which a piece of DNA is integrated into the genome of a host bacterium

(v) To produce higher yields of the desired protein, host cells can be multiplied in a continuous culture

(vi) Downstream processing is one of the steps of polymerase chain reaction

• A. (i), (iii) and (vi)
• B. (i), (iii) and (v)
• C. (ii), (iii) and (v)
• D. (i), (iv) and (v)

Answer 1

Answer: (A)

(i), (iii) and (vi)

Antibiotic resistance genes are selectable markers. Desirable genes are the ones which are introduced in the vector for getting desired protein product. In agarose gel electrophoresis, DNA fragments are separated according to their charge and size. After the formation of the product in a bioreactors, it undergoes through some processes before a finished product is ready for marketing. The processes include separation and purification of products which are collectively called as downstream processing.