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B
Alcohol
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C
Glycerine
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D
Xylene
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Solution
The correct option is A Glycerine
Mounting media are used for making permanent slides as it holds the specimens in place between the coverslip and the slide.
Glycerin- Jelly substance used in the mounting of tissues because it keeps specimens free from moisture and other contaminants and because of its high refractive index.
This may be necessary to see certain structures. If a lower refractive index is needed, then one should mix some water into the glycerin.