Question

Term 'Restriction' in restriction endonuclease enzyme refers to

• A. Cleaving of phosphodiester bond in DNA by the enzyme
• B. Cutting of DNA at specific position only
• C. Prevention of bacteriaophage multiplication in bacteria
• D. Cutting each of the two strands of DNA at specific points in sugar phosphate backbone

Answer 1

Answer: (D)

Cutting each of the two strands of DNA at specific points in sugar phosphate backbone

Restriction enzymes certain nucleotide sequences called restriction sites. They cleave the DNA near or at these restriction sites. They make a cut between sugar and phosphate components of a nucleic acid molecule.

So, the correct answer is 'Cutting each of the two strands of DNA at specific points in sugar phosphate backbone'.