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Question

The bacteria generally used for genetic engineering is

A
Agrobacterium
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B
Bacillus
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C
Pseudomonas
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D
Clostridium
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Solution

The correct option is A Agrobacterium
Agrobacterium tumefaciens is a soil plant pathogenic bacterium that carries Ti plasmid. It can transfer a particular segment of the tumor-inducing (Ti) plasmid into the nucleus of infected cells. The transferred T-DNA is then integrated into the host genome and transcribed with it. This ability of Agrobacterium tumefaciens to transfer the T-DNA in the host genome is explored in genetic engineering to transfer the desired DNA segment of up 25kb, carrying the gene of the interest, into the genome of selected organisms. Thus, the correct answer is option A.

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