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Question

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of:

A
Non-recombinant bacteria containing beta-galactosidase
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B
Insertional inactivation of alpha-galactosidase in non-recombinant bacteria
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C
Insertional inactivation of alpha-galactosidase in recombinant bacteria
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D
Inactivation of glycosidase enzyme in recombinant bacteria
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Solution

The correct option is A Non-recombinant bacteria containing beta-galactosidase
The blue-white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria . This technique is based on the insertional inactivation of the β-galactosidase gene.
Cells transformed with vector containing the recombinant DNA contain an inactive form of the β-galactosidase gene and hence produce white colonies.
cells transformed with non-recombinant plasmid i.e. only the vector, have an active β-galactosidase gene and thus produce blue colonies.

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