wiz-icon
MyQuestionIcon
MyQuestionIcon
1
You visited us 1 times! Enjoying our articles? Unlock Full Access!
Question

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of:

A
Non-recombinant bacteria containing beta-galactosidase
Right on! Give the BNAT exam to get a 100% scholarship for BYJUS courses
B
Insertional inactivation of alpha-galactosidase in non-recombinant bacteria
No worries! We‘ve got your back. Try BYJU‘S free classes today!
C
Insertional inactivation of alpha-galactosidase in recombinant bacteria
No worries! We‘ve got your back. Try BYJU‘S free classes today!
D
Inactivation of glycosidase enzyme in recombinant bacteria
No worries! We‘ve got your back. Try BYJU‘S free classes today!
Open in App
Solution

The correct option is A Non-recombinant bacteria containing beta-galactosidase
The blue-white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria . This technique is based on the insertional inactivation of the β-galactosidase gene.
Cells transformed with vector containing the recombinant DNA contain an inactive form of the β-galactosidase gene and hence produce white colonies.
cells transformed with non-recombinant plasmid i.e. only the vector, have an active β-galactosidase gene and thus produce blue colonies.

flag
Suggest Corrections
thumbs-up
1
Join BYJU'S Learning Program
similar_icon
Related Videos
thumbnail
lock
Process Of Recombinant DNA Technology
BIOLOGY
Watch in App
Join BYJU'S Learning Program
CrossIcon