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B
Insertional inactivation of alpha-galactosidase in non-recombinant bacteria
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C
Insertional inactivation of alpha-galactosidase in recombinant bacteria
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D
Inactivation of glycosidase enzyme in recombinant bacteria
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Solution
The correct option is A Non-recombinant bacteria containing beta-galactosidase The blue-white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria . This technique is based on the insertional inactivation of the β-galactosidase gene. Cells transformed with vector containing the recombinant DNA contain an inactive form of the β-galactosidase gene and hence produce white colonies. cells transformed with non-recombinant plasmid i.e. only the vector, have an active β-galactosidase gene and thus produce blue colonies.